Scripts: "it's open now."

A couple walks into the library, seemingly upper middle class, and requests access to the restrooms. They're inquiry is made short and quaint. "Excuse me, is it possible to gain access to the restroom?"

The reply from the employee is "It's open now." Does this reply differ in it's approach to accommodate a customer if it is annunciated with a comma? "It's open, now." I don't know why, but I get the sensation when using this phrase as a quick response to indicate to a customers I've just unlocked the door, as if I were talking to hyperactive children running around and causing a ruckus and trying to get their attention as they whiz by by saying "Careful! Don't knock down your little sister now."